Mice were allowed to explore the objects for 5 min, after which the mice were returned to their own cages for a 5-min retention period.
Mice were allowed to explore the objects for 10 min, after which the mice were returned to their home cage.
The control mice were returned to their home cages immediately after pairing training and left undisturbed.
Echocardiography was performed under a brief period of isoflurane anaesthesia before and immediately after re-warming, and then the mice were returned promptly to their metabolic cages where oxygen consumption recordings were resumed within 1 to 2 min.
40 mice were returned to the island in late 2006 with the remaining being re-introduced in stages.
Thereafter, mice were returned to their new testing cage.
One day after conditioning, mice were returned to the chamber.
Following stress, mice were returned to their home cages.
Following infection mice were returned to cages, bled periodically, and observed for symptoms of disease.
On day 8, mice were returned to normal drinking water and sacrificed on day 10.
Subsequent to the challenge, mice were returned to the standard chow diet and group housing.
