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The mice were restricted from food and water four hours prior to each inoculation to reduce variation in systemic invasion due to food in the gut.
Although there were relatively long-term differences in absolute body weights, an analysis of body weight changes over the P2 P14 period showed that significant losses of body weight in the PCP-treated mice were restricted to the first post-treatment day (data not shown).
Interestingly enough, the transgenic expression of HLA-A2 molecules did not improve the rates of human CD8 T cell reconstitution as compared to non-transgenic littermates, though the human CD8 T cells developed by some of the transgenic mice were restricted by HLA-A2 molecules.
Tumours in AOM-treated mice were restricted to females and were localized exclusively to the distal colon.
The mice were restricted from food and water four hours prior to inoculation to rule out variation in systemic infection due to food in the gut.
Prior to the start of training, mice were restricted to 2 3 g of dry food per day, with unlimited access to water.
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Food intake of diet-restricted ob/ob and DKO mice was restricted to 2.5 g/day, which is the normal daily intake of lean WT mice, between 12 and 24 weeks of age [ 11].ACE-inhibition was obtained with captopril (10 mg/kg/day) intraperitoneally from 12 until 24 weeks of age in WT, ob/ob and DKO [ 22].
These results suggest that insulin signaling defects in dmpk−/− mice are restricted to DMPK-expressing tissues.
The increased susceptibility of immunized mice was restricted to peripheral prion exposure, and was not observed after i.c.
As reported in Fig. 4, all groups of mice generated a comparable TRP-2-specific CTL response, therefore confirming that tolerance in TRAMP and TRAMPJα18−/− mice is restricted to the PC-associated antigen.
As increased physical activity in KRAP−/− mice is restricted to dark period (Figure 2B), the higher physical activity does not fully account for the increased whole-body energy expenditure, suggesting that other processes such as heat production will be involved in the altered energy homeostasis in KRAP−/− mice.
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