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For the tail flick test, mice were restrained in a tube with only it's hind legs and tail protruding.
To apply the stimulus, mice were restrained by hand in a supine position and slowly stroked with the stimulus until it bubbled at the nostrils, stroked again, and replaced in the cage.
Conscious mice were restrained, and the sensor was placed at the base of the tail.
The mice were restrained from food and water intake for 1 hr after inoculation.
For lung irradiation, anesthetized mice were restrained in modified Perspex tubes.
In order to make the hole the mice were restrained for 20 s.
Similar(40)
While control animals (CON, n = 8) were left undisturbed in their home cages, one group of mice was restrained in a plastic tube for 30 minutes (RES, n = 8).
For each treatment, the mouse was restrained by hand and the LED was held 1 to 2 cm above the head.
Prior to imaging, the mouse was restrained using a home-made mount and the hind limbs were positioned as shown in Fig. 1(C).
Each mouse was restrained in a Perspex chamber and acclimatized to background white noise of 70 dB SPL for 1 minute.
Mice in the EA group were physically restrained, then electro-acupunctured on Zusanli (ST36) and Neiting (ST44), while mice in the control group were restrained in the same way, without acupuncture.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com