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RCS-SCID mice were rested for 4 wks prior to MPTP intoxication.
All mice were rested at least 10 14 days before starting acclimations and baseline recordings.
After vaccination, the mice were rested for sufficiently long-duration (240 days) before challenge with M. tb, to establish bona fide T cell memory response [18].
The recipient mice were rested for 6 weeks, infected with vv and 3 days later their PEL examined for the presence of donor-derived Thy1.2+ CD8 T cells.
To explore this issue, Ad-CMVpoly immunized HHD-2 mice were rested for one or three months and then immunized with the Ad-CMVpoly vaccine (7.5×108 pfu/mouse).
The mice were rested for 2 days.
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12, 13 EEG and video recordings were obtained while mice were resting in a state of quiet wakefulness.
Rectal body temperature in wild-type and hadh−/− mice was measured with a rectal thermometer for mice (physitemp BAT-12; Physitemp Instruments, Clifton, NJ) when mice were resting.
The orbital blood from each mouse was rested for 30 min at room temperature and spun at a low speed (1000 rpm) for 15 min at 4°C.
The selected mouse was rested for 1 month followed by intravenous boosting with 50 μg of KLH conjugated immunogenic peptide in 100 μl 0.9% sodium chloride solution 3 days before isolation of the spleen for cell fusion.
After the fourth immunization, this mouse was rested for one month and then boosted intravenously with 50 μg of CO4-MMP in 100 μL of 0.9% sodium chloride solution three days before isolation of the spleen for cell fusion.
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