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Plasma samples from tnc +/+ and tnc− / − mice were resolved by SDS-PAGE and analyzed by western blotting using goat anti-tenascin-C polyclonal Ab (AF3358; R&D Systems).
Low numbers (1 2) of histopathologically inconclusive mice in five tissue homogenate groups, which included two groups (A1212, popliteal and submandibular lymph nodes) that contained no histopathologically positive mice, were resolved almost exclusively as negative when examined using immunohistochemistry.
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Samples (50 µg protein for HEK293 cell lysates, 50 µg protein for whole retinal lysates, and 50 µg protein for mouse retina membranes) were resolved by SDS-PAGE on 8 10% acrylamide gels [17].
Mouse tissue samples were resolved by SDS-PAGE and transferred to nitrocellulose membrane (Invitrogen), followed by specific primary antibodies and HRP-conjugated secondary antibodies.
Mice treated with JAKi had mild inflammation of the paws, except for the toes and fingers, for a week only, as clinical signs were resolved in all mice after 17 days of treatment.
Twenty-five to fifty µg of total protein from mouse epidermis or keratinocytes (control and UVB treated) were resolved using SDS-PAGE.
Equivalent amounts of protein were resolved on 10% (rat) or 8% (mouse) SDS-PAGE and electrotransferred to a nitrocellulose membrane.
Proteins were resolved by 12% SDS-PAGE and blotted with a mouse monoclonal anti-PDI (1D3 Assay Designs, Exeter, UK).
The mouse gradually returned to its initial weight and approximately 2 months after surgery the burns were resolved.
Those issues were resolved.
Any queries were resolved.
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