In all regimens, corneal epithelia of narcotized mice were removed by a hockey epithelium removal knife prior to PDI.
The brains of the mice were removed, frozen, and embedded in Optimal Compound Temperature compound (Sakura Finetek, USA).
Following the end of the conditioning session, mice were removed from the conditioning chamber, placed back into their home cage and returned to the habituation room.
Several weeks into their development, the tumors from both groups of mice were removed and subjected to DNA extraction by lysis of tissue samples.
In separate studies, mice were removed from their metabolic cages at 10 and 24 h and then re-warmed in a heating chamber to 37°C (rectal temperature) over 1.5 h.
After 60 seconds the mice were removed to a drying cage as before.
The ON, SC, and brain of infected mice were removed at necropsy on day 14 PI.
Tibias and femurs of tumor bearing mice were removed and flushed with PBS.
For skeletal analysis, skin and internal viscera of E18.5 embryos and newborn mice were removed.
administration in this paradigm, habituated mice were removed from the experimental chamber, briefly restrained, i.c.v.
To assess bone microarchitecture, the right femurs of the mice were removed and cleaned of all soft tissue.
