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Thereafter, mice were regularly checked on pain stimuli and discomfort.
Tumor sizes and body weights were recorded twice weekly, and the mice were regularly observed over the course of the study.
During the intervention, the mice were regularly monitored for weight, food consumption, and physical endurance.
The incision was closed using Vetbond (3M) and mice were regularly monitored throughout the course of infection.
The mice were regularly examined, tumor sizes were measured with a caliper, and tumor volumes were determined using the following formula: volume = 0.5 × (width)2 × length [ 26].
DBA/1 mice were regularly weighed from the day of priming and checked for the development of arthritis after booster immunization.
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Blood of the transplanted mice was regularly collected from the tail vein.
However, male house mice are regularly exposed to a risk of sperm competition within their polygynous mating system because females often seek extra-territorial copulations that can result in multiply sired litters (Dean et al. 2006).
The mice were also regularly injected with luciferin, so that the luciferase would have the fuel it needed to glow.Because resulting light was so dim, Dr Leib worked with Gary Luker and David Piwnica-Worms at Washington University, St Louis, to place the animals in a dark box and photograph them using a special camera.
The mice were monitored regularly for tumor growth.
All infected mice were monitored regularly by daily weighing and assignment of health scores.
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