B6 mice were reconstituted with BM cells from either B6, Tga20 or Prp0/0 mice; Tga20 mice reconstitution was performed with Tga20, B6 or Prp0/0 BM cells; and Prp0/0 were reconstituted with BM sampled from Tga20, B6 or Prp0/0 mice.
T cell deficient CBA Rag1 /– mice were reconstituted i.v. with syngeneic fractionated T cells.
SCID mice were reconstituted with i.v. injections of 8×107 unfractionated SPC populations from WT B6 mice.
In addition, all mice were reconstituted with high levels of human CD4+ T cells in peripheral blood (Figure 1C).
Each set of Prp0/0, Tga20 or B6 congenic mice were reconstituted with femoral BM from the three same sources.
B cell deficient µMT mice were reconstituted with a mixture of 75% µMT/25% WT bone marrow or 75% µMT/25% MHC II−/− bone marrow.
To explore this possibility, we constructed bone marrow chimeras in which lethally irradiated C57BL/6 mice were reconstituted with allogeneic BALB/c bone marrow.
As a positive control, Rag1−/− mice were reconstituted with 107 total splenocytes from naïve C57BL/6 mice prepared as describe above.
Following irradiation the mice were reconstituted with 2×106 bone marrow cells that were depleted of T cells using anti-CD90 (Thy1.2) (Miltenyi Biotec) magnetic bead separation.
NOD-SCIDβ2m-/ mice were reconstituted intra-peritoneally with human HLA-A*0201+ HLA-A*0201+ekly vaccinated subcutaneously with irradiated PBMCide-pulsed GEN cells before or ander being challenged weeklyelanoma tumor cells.
Thus, 14 out of 15 (93.3%) DRAG mice and 9 out of 11 (81.8%) control mice were reconstituted with human B (CD19+) cells by 10 weeks after HSC infusion (Fig. 5A).
