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Body weight and tumour volume of all mice were quantified 3 times per week.
The tumor sections with immunopositive regions from control and treated mice were quantified for intensity, size and area using Ariol software Review Station.
GC levels in liver, lung, and spleen from 9 V/null and WT mice were quantified by LC/MS-MS (Lipidomic Core, University of South Carolina).
The absence of effects on adiposity between control and LON mice were quantified by determinations of body weight and composition as shown in Fig. 1B and Table 1.
Trophic shifts of the mice were quantified by the differences in the proportional contributions of each trophic group to the mouse diets between populations that received resource pulses and those that did not.
The BLI signal intensities emitted from the liver region of the mice were quantified using a fixed region of interest (ROI) of 2×1.5 cm in size from dorsal images and expressed as photons per second of light.
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Latent viral load in the spleens of infected mice was quantified by real-time PCR using the ABI 7300 Real Time PCR System (Applied Biosystems, Foster City, CA, USA).
Latent viral load in the spleens of infected mice was quantified by real-time PCR using the ABI 7300 Real Time PCR System (Applied Biosystems, Foster City, CA).
Interaction between the male and female mice was quantified by manual annotation of video recordings in which the behaviors were categorized as sniff body, approach, chase, walk away, mount and attempt mount, thrust and/or ejaculation, or non-interacting.
The degree of vascular albumin leakage from cremasteric venules of Mac-1-/ and wt mice was quantified as described previously by both our laboratory [18] and others [19], [20].
Analysis of the ambulatory gait of mice was quantified using the DigiGait Imaging System (Mouse Specifics, Inc).
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