When castrated long day-housed mice were provided with long day levels of testosterone, evoked GnRH release was restored to intact levels.
Adult (3 to 6 months old) and older (22 to 24 months old) male mice were provided with either a standard diet or diet containing 6 grams luteolin/kg.
The mice were provided with pelleted protein-enriched diet and water ad libitum.
For the CR group, ability to move was recorded before mice were provided with their daily food portions.
For the CR group, clinical score was recorded before the mice were provided with their daily food portions.
Indeed, when mice were provided with a highly palatable diet they responded with conspicuous overfeeding as previously observed with cafeteria diet [78].
The CR mice were provided with food equivalent to 60% of the average intake of the AL group on a daily basis.
For control treatments, mice were provided with a standard diet containing only 6% fat (Cat. No. 5K52, Lab Diets, St . Louis MO).
In accordance with the "Animals for Research Act of Ontario" and the Guidelines of the Canadian Council on Animal Care, mice were provided with the healthiest conditions and most humane care practically possible.
To exclude or minimize other exogenous sources of estrogenic agents, mice were provided with certified phytoestrogen-free rodent diet (TD96155; Harlan Teklad) and provided tap water ad libitum throughout the experiment.
Group 2 mice were provided with free access to regular chow and with restricted access to a supplementary palatable meal at ZT 4 00 6:00 each day for 67 days.
