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In contrast to the gene trap mutants, no homozygous gene-targeted mice were present at parturition; thus, embryos were analyzed at different developmental stages.
Significant numbers of host reactive Vbeta3+ T cells (3.56+/-1.66% among CD4, 4.06+/-1.62% among CD8, P<0.0001 vs. normal BALB/c mice, P>0.05 vs. normal B10.D2 mice) were present in PG490-88-treated mice, suggesting that clonal deletion was not responsible for the observed tolerance.
The T-cell epitope peptides of BALB/c mice were present in four regions of 3 to 5 overlapping sequences comprising 16 of the 19 total epitope peptides (Figure 1; Table 1).
The fact that i) bacteria represented by the upper band completely absent in TLR4-/ mice were present in the luminal colon contents from TLR4-/ animals that grew up in another facility (Figure 3B, black arrow), and ii) that the lower band was detected in one of the TLR4-/ animals (Figure 3B) provided strong evidence that these differences are most likely caused by interindividual variability.
Cdo –/− ;Boc +/− mice were present at slightly less than the expected frequency (supplementary material Table S1).
Female mice also vocalized when freely moving male mice were present.
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Twenty-four hours following the completion of reversal training, mice were presented with a second probe test.
C57BL/6 mice were presented with either water only or a choice of water and a 10% v/v ethanol solution.
Next day, during acquisition phase, mice were presented with two identical objects (object A + object A) in the same open-field environment for a total duration of 3 min.
The second employed a multiple bottle-choice version of the DID procedures, in which AAV-infused mice were presented with 5, 10, 20 and 40% (v/v) alcohol for 1 week, followed by 5, 10 and 15% (w/v) sucrose for 1 week.
We next conducted a third replicate of the study to address the possibility that Homer2b knockdown in the CeA altered alcohol/sucrose sensitivity, in which the mice were presented simultaneously with 5, 10, 20 and 40% alcohol or with 5, 10 and 15% sucrose for 2 h daily over the course of 7 days.
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