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(G) BMDMs from the RBP-J cKO or control mice were plated into the upper chamber of 24-well transwell chambers.
After 5th day of infection, homogenized samples of liver, spleen and MLN of infected mice were plated on antibiotic plates and the colonies were counted.
Thyoglicolate-elicited peritoneal macrophages collected from BALB/c mice were plated and non-adherent cells were removed by washing in Hank's Balanced Salt Solution (HBSS - Sigma-Aldrich Co, St . Louis MO, USA) after 2 h incubation at 37°C.
For cell adhesion assay [25] and time lapse imaging of attached keratinocyte, equal number of keratinocytes from knockout and control mice were plated per well of ColI/FN coated 12-well tissue culture plates.
Osteoblasts harvested from calvarial of Pyk2+/+ and Pyk2−/− mice were plated as above on glass slides or in 6 well plates and exposed to both methods of FF for 30 minutes or maintained in static culture conditions.
FACS data were analyzed by FlowJo software (Tree Star Inc .. Dissociated primary mammary cells from C57BL/6 mice were plated on collagen I-coated plates (BD Bioscience) in mouse mammary epithelial cell cultured media.
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One milliliter of splenocytes, at a concentration of 1 × 106 cells/mL (from aged B6C3F1 mice) was plated in each of six wells of a 24 well plate.
As a control, blood from non-tumor-bearing mice was plated as well, and absence of epithelial tumor cells was confirmed.
Seven days after differentiation, macrophages from each mouse were plated into 2 wells of a 6-well dish at 2.5 million per well in RPMI containing 5% heat-inactivated FBS.
Macrophages from each mouse were plated in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum and 1 100 penicillin/streptomycin. Nonadherent cells were removed after 4 h, and the medium was replaced with fresh medium.
To determine the fungal load in the vagina, 50 μl of the lavage fluids from each mouse were plated on YPD agar plus chloramphenicol (50 μg/ml), then CFUs were evaluated.
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