At the end of adenanthin treatment, mice were killed and tumors were picked, photographed, size measured and weighed.
EpRas clonal lines were selected in 3 µg/mL puromycin for two weeks after transfection with control shRNA or shRNAs to human and mouse MRP (Open Biosystems), colonies were picked and maintained in media with puromycin.
Resistant colonies were picked onto mouse embryonic fibroblast-coated 24-well plates, propagated and analyzed for mCh expression.
For induced pluripotent stem cell characterization, GFP+ embryonic stem cell-like colonies were picked up and passaged on irradiated MEFs in mouse embryonic stem cell medium.
These random values were picked from ranges determined from the available data from wild-type mice.
Mouse ES cells were transfected with the linearized targeting vectors and neomycin-resistant ES cell clones were picked and screened.
Colonies were picked and stocked.
Selected spots were picked with a 2 mm picking head.
were picked for maturity analysis.
Clones were picked based on morphology.
