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Following the injections, mice were permitted to recover and monitored daily, including tumor measurement using calipers.
Specifically, RB-induced lesions in both control and test mice were permitted to expand to 0.95±0.17×105 µm2 (n = 3) and 0.89±0.12 µm2×105 (n = 3) for 24 hours, respectively (Fig. 3).
Mice were permitted to eat and drink ad libitum and were fed standard mouse chow (Cargill Agri Purina, Inc., Seongnam-Si, Korea).
Mice were permitted to recover for at least 1 week, before the experimental sessions began.
Mice were permitted to recover from surgery in a pre-warmed cage.
Mice were permitted 1 week to acclimatize to their environment before being manipulated and used at 8 weeks of age in all experiments.
Similar(53)
Since even modest handling of mice may induce alterations in heart rate [ 6], each mouse was permitted to acclimatize for 10 min prior to collection of baseline data.
Afterwards, we placed the mouse in the gustometer; the training session began when it took its first lick and lasted for 30 min. On each training day, the mouse was permitted to drink water freely from a single stationary spout during the training session.
No ladies were permitted to….
Tied ranks were permitted.
Nighttime campfires were permitted.
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