To maintain fungal virulence, serial passages in C57BL/6 mice were performed before the isolate H99 was used in experiments.
Virulence and cell analysis based immunological assays using C57BL/6 and/or SCID mice were performed as described66,67.
In utero electroporation of C1qa KO mice and C3 KO mice were performed at E14 with 7 mm electrodes (39 mV pulses).
To further assess whether or not PET counterpart [18F]-9 atministracerat traconcentrationsions relevant for nuclear imaging demonstrates optimal kinetics (signal/noise ratios) to enable brain imaging in vivo, quantitative biodistribution studies in normal mice were performed.
All procedures involving mice were performed in accordance with guidelines set forth by the American Association for Accreditation of Laboratory Animal Care and the USPHS Policy on Humane Care and Use of Laboratory Animalss".
Inoculation with GCSCs and treatment in BALB/c nude mice were performed as described above.
All the experimental procedures in mice were performed in compliance with the protocol approved by the Institutional Animal Care and Use Committee (IACUC) of Tsinghua University.
Thus, to evaluate the potential of radioiodinated -p IV for tumor imaging, biodistribution experiments of -125I]p IV using tumor-bearing mice were performed.
For assessing its overall pharmacokinetic profiles in general and its ability to cross the blood-brain barrier (BBB) in particular, biodistribution studies in normal mice were performed.
SUVbrain comparisons before and after P-gp inhibition for each animal group separately (i.e., rats, Mdr1a/1b dKO, or WT mice) were performed using paired samples t tests, again with Bonferroni correction to adjust for multiple comparisons.
Breeding and maintenance of mice were performed under institutional guidelines.
