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By day 7, these mice were moribund (Fig. 1B).
For the SFTPC-Cre; Rosa26RSox2-IRES-GFP/+ experiments, three mice were moribund at postnatal day (P) 11 and one at P16.
In addition, all αLy-6G treated mice were moribund by Day 2 post-inoculation, indicating that B. pertussis-specific sera did not protect against the rapid virulence of B. bronchiseptica in animals lacking neutrophils.
Mice were sacrificed when the tumor size exceeded 15 mm in diameter in either direction or when the mice were moribund or at the end of the observation period (50 d).
The experiment ended when mice were moribund.
The mice were moribund 2 days later and had extremely high spirochetemias.
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Mice were acclimated for 4 days prior to receiving a single 200 μg dose of rHIgM12 or control human IgM that does not bind to the surface of neurons at 60 days of age, and were then continuously monitored until the last mouse was moribund.
Kidneys were obtained from mice that were moribund or at the end of an experiment, visually examined for abscess formation, and homogenized for CFU enumeration.
Expression of luciferase in brain, lungs, spleen and liver was analyzed in non-perfused, dissected organs from mice that were moribund.
21, 22 Remarkably, MT expression levels in treated animals were significantly lower than the levels measured in untreated age-matched GLD mice, which were moribund at this disease stage (see Fig 4A).
This curious finding suggests a lack of congruence between splicing abnormalities and cellular pathology, and, given that the mice assayed were moribund, might simply reflect a non-specific response to tissue injury.
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