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Both female and male mice were mixed in each group.
Bone marrow cells from CD45.2+ IκBα-SR Tg mice were mixed with equal cell numbers from CD45.1+ wild-type mice and intravenously injected into lethally irradiated Rag1−/− mice.
Bone marrow cells from the femurs of 6-week old CD45.2+ IκBα-SR Tg (1.5×106) and CD45.1+ C57BL/6 (1.5×106) wild-type donor mice were mixed at a ratio of 1∶1.
Bone marrow cells from WT and the Siglecg−/− mice were mixed at a 1∶1 ratio (2.5×106 each) and were injected into lethally irradiated (1100 RAD) WT or Siglecg−/− mice.
FACS-purified LSK cells from wild-type and Nfil3 −/− mice were mixed at a 1 1 ratio and transplanted into recipient mice by retro-orbital injection.
In order to have a uniform cell population between replicates at each instance, the muscle cell homogenates from different mice were mixed well and the resulting cell mixture was used to prepare the cultures.
Similar(29)
c-kit positive ICC, or pacemaker cells, have been convincingly demonstrated in bladders from a number of species including human [47] [48], dog [49], pig [50], and guinea pig [51] [52], however studies on bladders of mice are mixed.
Another 0.1 μg miRNA from corn oil-treated mice was mixed with the same reagents, except for Cy3 instead of Cy5.
To determine the self-renewal ability and multilineage potential of HSCs, we used competitive repopulation (Harrison, 1980), in which donor BM cells of either WT or Smurf2-deficient mice (CD45.2+) were mixed at a 1 1 ratio with competitor BM cells of 2-month-old CD45.1+/CD45.2+ WT mice and injected into lethally irradiated congenic recipient mice (CD45.1+).
BM cells from Cav-1+/+ or Cav-1−/− mice (CD45.2+) were mixed at a ratio of 1 : 1 with competitor BM cells (CD45.1+45.2+) and injected into lethally irradiated CD45.1+-recipient mice.
In brief, hemagglutinin, mouse anti-6X His IgG (Abcam), and RPE-conjugated goat anti-mouse Ab were mixed in a ratio of 4 2 1 and kept on ice for 20 min. The resulting precomplex was then topped up with assay buffer to a final HA concentration of 4 μg of HA per assay.
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