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Subsequently, the animals were orally intubated, an arterial line was placed in the arteria carotis, and the mice were mechanically ventilated (MiniVent®, Hugo Sachs Elektronik-Harvard Apparatus, March-Hugstetten, Germany).
Histopathology of lung tissues showed that major airways of four mice were mechanically blocked by the GO conglomeration, which led to suffocation in 15% of the GO-exposed mice, and was not evidence of pulmonary toxicity of GO.
The splenocytes of six M.fortis, six rats, and six mice were mechanically fragmented and placed in a petri dish containing 5 mL RPMI1640 medium supplemented with 10% fetal calf serum.
C57/Bl6 mice were mechanically ventilated.
Neurospheres derived from eGFP mice were mechanically dissociated into cell suspension.
Fresh xenografts of S1-M1-80 S1-M1-80 S1-M1-80rom nude micellsre mechanically diseparateded with sterile crossed scalpels to obtain pieces ofromout 1 mm.
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Briefly, complete pancreas from 8-week-old mice was mechanically and enzymatically digested with collagenase to obtain isolated acinar structures.
The role of mechanical stress in stimulating myofibroblast activity has also been shown in experiments where dermal wounds in mice are mechanically stressed by stretching or splinting the wound, where increased myofibroblast activity results in increased scar formation, to some extent mimicking hypertrophic scarring that is seen in humans.
Relative RNA expression of inflammatory markers in lung homogenate after 12 h of mice that were mechanically ventilated with high tidal volumes compared to controls.
The RNA expression of selected markers showed an increased relative expression of TNF-α, IL-6, and MCP-1 in lung homogenate of mice that were mechanically ventilated with high tidal volumes compared to controls (Additional file 1 Figure 6).
For isolation of splenocytes, mouse spleens were mechanically disrupted and red blood cells were lysed.
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