We dedicate this work to the memory of S. Korsmeyer, in whose laboratory the Tlx gene mutant mice were made.
In another experiment, adult male C57BL/6J mice were made dependent using the intragastric alcohol consumption (IGAC) procedure.
Importantly when BCG-treated normal mice were made diabetic with STZ, a chemical that destroys pancreatic insulin-secreting cells, blood sugar reductions of at least 30% were achieved.
Adult male FVB mice were made diabetic with a single injection of STZ (200 mg/kg, i.p .. Fourteen days later, control and diabetic (fasting blood glucose >13.9 mM) mice received benfotiamine (100 mg/kg/day, i.p).
Adult male wild-type (FVB) and MT transgenic mice were made diabetic by a single injection of streptozotocin (STZ).
The oral and colon tissue from the mice were made into tissue lysis by RIPA to do further study.
Three groups of mice were made, a sham group without sepsis, a control group (LPS-NaCl) and an urantide-treated group (LPS-urantide).
Comparisons of measurements between groups of mice were made using Student's unpaired T test with an alpha level of 0.05 considered significant.
Metabolic measurements in mice were made over 24 h in six controls, six with mild sepsis and seven with severe sepsis.
Sure enough, when the boosted mice were made to navigate a maze in order to get a reward of chocolate milk, they learned the route faster than the mice without the added human gene.
At 6 weeks of age, mice were made diabetic via 5 consecutive daily doses of streptozotocin (Sigma, 50 mg/kg in citrate buffer, pH 4.5; St . Louis MO, USA).
