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Eight week-old male C57BL/6 mice were lightly anesthetized.
First, female bone marrow transplant recipient mice were lightly anesthetized with isoflurane.
For viral and bacterial challenge, 7 to 9 week old mice were lightly anaesthetized by isofluorane inhalation.
Adult mice were lightly anesthetized with 2,2,2-tribromoethanol then photographed live (Canon Powershot SD600, Canon, NY, USA).
Twenty-four hours after passive immunization, mice were lightly anesthetized by inhalation of CO2 and challenged intranasally (i.n).n
After six hours of fasting, mice were lightly anesthetized and injected intraperitoneally with 2 mg glucose/g body weight.
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A total of 50 μl containing 5 × 10 colony-forming units (cfu) were inoculated intranasally in mice which were lightly anesthetized by inhalation of isoflurane (Upjohn, Ede, the Netherlands).
BALB/c mice (Charles River, United Kingdom) were lightly anesthetized with 2.5% (vol/vol) isoflurane over oxygen (1.8 to 2 liters min−1) and infected intranasally with 1 × 10 mycobacteria.
Swiss Webster mice (Taconic) were lightly anesthetized with isofluorane and administered mixtures of VT750-fibrinogen (1 nmol by VT750) with thromboplastin (n = 3 mice per dose) via tail vein injections.
4- to 5-week-old C57BL/6 mice from Taconic were lightly anesthetized by intraperitoneal injection of ketamine/xylazine (35 100 μg ketamine/gram body weight), and sporozoites were injected intradermally into the dorsal sheet of the ear pinna in a total volume of 0.2 μl using a NanoFil-10 μl syringe with a NF33BV-2 needle (World Precision Instruments).
Prior to performing the injections into tumors, mice were first lightly anesthetized with isoflurane gas.
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