After the last injection, adolescent-treated mice were left undisturbed until adulthood (Figure 1a); adult-treated mice were left undisturbed for at least 10 days after the final injection to allow WIN to wash out of tissue.
The mice were left for two weeks to acclimatise before being injected with tumour cells.
Next, after a similar pulse of tamoxifen, mice were left for a latency period of 14 days before being inoculated subcutaneously with B16-F0 cells.
One mouse fully recovered from its TTS; 10 mice were left with PTSs at all frequencies; 26 mice recovered at some frequencies but not others.
Interventions: In group 1, mice were left untreated; in groups 2 through 5, a well-defined area on the dorsum was exposed to 20Gy x-ray irradiation.
The influence of the pharmacological treatment over the studied parameters was discarded since four additional mice were left uninfected, treated with pzq as described above and analyzed as the other animals.
For the memory T cell experiment, male C57BL/6 mice were left untreated or treated with a dose of 4 × 1011 vg kg−1 of an AAV8-RFP vector.
In an additional set of experiments, male C57BL/6 mice were left untreated or treated with AAV8-Luc vector (4 × 1012 vg kg−1) together with SVP[Rapa] or SVP[empty].
Both when the mice were left on their own and when they had any of four female researchers in the room with them, the injections induced visible distress.
LL2 tumour bearing mice were left untreated, or treated with chemotherapy (chemo) and/or RIT 177Lu-DAB44 177Lu-Sal5al5).
The mice were left to equilibrate for 15 min before baseline values for HR, sBP, SpO2, shock index, EF, CO, and SVR were determined.
