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Briefly, splenocytes and lymph node cells from DO11.10 mice were labeled with CFSE and a cell suspension containing 2.5e6 CFSE-labeled DO11.10 CD4 T-cells was then injected into the tail vein of BALB/C recipients.
Splenocytes from immunized mice were labeled with CFSE as described above.
When indicated freshly isolated CD4+ T cells from 2D2 mice were labeled with the vital dye CFSE (Molecular Probes).
BM-DCs generated from B6.Sjptprca (H2b, CD45.1+) mice were labeled with 1.5 µM CFSE, and 10×106 BM-DCs were injected i.v. in C57BL/6j mice.
The vitamin E supplemented keratinocytes isolated from control and knockout mice were labeled with 75Se to visualize the expression of selenoproteins.
To monitor the expansion and proliferation of HIV-specific T cells, splenocytes from HIVBr18 or pVAX1 immunized mice were labeled with carboxyfluorescein succinimidyl ester (CFSE) [56].
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To investigate this possibility, wild-type and Pvrl1−/− mice were labelled with BrdU and their enamel organs examined.
All samples were labelled with Cy5, and 3 samples of unexposed Wt mice were labelled with Cy3, pooled and used as a reference sample.
Spleen cells from B6 mice were labelled with biotinylated antibodies against Thy1.2 53-2.1 53-2.1mingen), followed by labelling with strePharmingenonjugated paramagnetic microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany).
Spleen cells from B6 mice were labelled with biotinylated antibodies against CD4 (GK1.5; Pharmingen) and CD8 53-6.7 53-6.7mingen) followed by labelling with strePharmingenonjugated paramagnetic microbeads (Miltenyi Biotec).
More importantly, tau inclusions in the spinal cord of human P301S tau transgenic mice were labelled following a single intravenous injection of FSB.
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