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The mice were kept on a 12-h light dark cycle.
(a d) Six-month-old WT and Sesn2−/− mice were kept on HFD for 4 months.
All mice were kept in specific pathogen-free facilities at Keio University.
After 10 days, primary cutaneous tumors were excised and mice were kept alive to progress to metastasis (Fig. 7a).
The mice were kept anesthetized and fixed in the animal bed as for FMT.
After injection, the mice were kept sedated on a heating pad for about 40 min.
The mice were kept warm during the scan using an infrared lamp.
Pairs of mice were kept in type II cages (16 × 22 × 14 cm3 (width × length × height); Tecniplast, Hohenspeissenberg, Germany).
Immunodeficient mice were kept in sterile isolators.
All mice were kept on C57BL/6 background.
Trangenic mice were kept in C57Bl/6 background.
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