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Furthermore, adipocyte precursors from wild-type mice were isolated, FACS sorted and infected with control (Ctrl) or lentivirus driving doxycycline-inducible expression of Flag-tagged LSD1 (Fig. 8b).
To investigate cell autonomy of LSD1 in Tg mice, adipocyte precursors from the SVF of Tg and control mice were isolated, FACS sorted and induced to undergo adipogenesis.
The cortex and cerebellum of two 24-week-old female C57BL/10cSnJ wildtype mice were isolated and snap frozen on dry ice.
Splenic CD4 T cells of NZB mice were isolated and stimulated by co-culture of T cells with individual band 3 peptides.
Mid brain from mice were isolated and homogenized in RIPA buffer containing protease inhibitors.
The islet cells from pancreas of NOD mice were isolated as described in Methods.
Spleens from BCG infected mice were isolated after 30 days of infection.
Ovaries from 16 week old mice were isolated and paraffin embedded.
Hepatocytes from gp130– and gp130flox mice were isolated as described previously [24].
Splenic macrophages from Tg+ sick mice were isolated by magnetic separation as described above.
Brain samples from C57 BL/6 mice were isolated from anesthetized and perfused animals[63].
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