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The mice were inspected and weighted daily for the assessment of ascites development.
Briefly, mice were inspected for illness or tumor formation twice every week.
To assay the appearance of mammary tumors, the mice were inspected three times a week and were euthanized when tumors appeared.
To determine survival rates, mice were inspected for vitality twice a day, and individuals showing ∼15% weight loss, neurological abnormalities or extreme anorexia were sacrificed immediately according to the animal protection regulations of the Duke University Medical Center Animal Care & Use Program.
The mice were inspected daily and weighed at weekly intervals.
After implantation, mice were inspected twice a week.
Similar(22)
Frozen human and mice tumoral samples were inspected by haematoxylin-eosin staining for epithelial tumour content by analysis of two slices at each end of the tumour.
The surfaces of the lungs of the mice in the prevention experiment were inspected for the presence of metastatic 4T1 tumors.
At week 40, mice were killed and colons and livers were inspected for macroscopic pathological lesions.
Necropsies were performed on all mice, and regional lymph nodes and internal organs were inspected for metastases.
The scores plots of PC1 versus PC2 and PC1 versus PC3 were inspected for differences between the different mouse strains.
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