To assess metabolic gas exchange, mice were individually housed in indirect calorimetry cages (CLAMS, Columbus Instruments).
After 1 h, mice were individually placed in the center square facing either one of the open arms.
All mice were individually weighed, were anesthetized with 2% Phenobarbital (60 ml/kg, i.p ., and then were sacrificed through cervical dislocation.
Three WT and four BCS mice were individually used for the Large PreB cell sorting.
Upon weaning, female mice were individually caged in a room without males.
Subsequently, mice were individually housed for two days and then perfused with 1.25% glutaraldehyde and 0.5% paraformaldehyde.
Habituation: Test mice were individually allowed to freely explore the entire apparatus for 25 min the night prior to testing.
Briefly, mice were individually put into a shock chamber and were allowed to freely explore the chamber for 2.5 minutes.
Briefly, during training session, mice were individually put into the shock chamber and were allowed to freely explore the environment for 150 seconds.
Typically, 6 10 week old mice were individually housed in wheel running cages placed inside light tight boxes with independent illumination.
For high-fat diet treatment, mice were individually placed on a fat-adjusted diet (60 kcal% from fat, Research Diets, Inc., New Brunswick, NJ) for 8 weeks.
