Alternatively, splenic T cells isolated from naive mice were incubated together with WT or March1−/− BMDCs at 37 °C 5% CO2 for 24 h.
For Sm-TNF-KO experiments, viable cremaster arteries from untreated or TAM-treated mice were incubated at 60 mm Hg in MOPS.
Hippocampal slices from control and SPLfl/fl/Nes mice were incubated in the absence (black traces) or presence (red traces) of 15 μM epoxomicin (Epoxo) for 5 h.
Purified spleen CD8+ CDCsc+ DCs and bone marrow-derived DCs (BM-DCs) from WT or 3d/3d mice were incubated for 2, 4, or 6 h with different concentrations of OVA-coated beads (OVAb).
Submandibular glands from ICR mice were incubated in vitro at 37 °C in modified Krebs-Ringer medium containing 0.2% glucose, and bubbled with a gas mixture of 95% O2 and 5% CO2.
ASCs isolated from green fluorescence protein (GFP) transgenic mice were incubated in an adipogenic medium and then seeded onto type I collagen sponge, non-woven polyglycolic acid or hyaluronic acid gel.
Macrophages isolated from heedless, oblivious, insouciant and Tlr1−/− mice were incubated with different concentrations of LTA for 4 hours.
No signal was elicited when islets from mceph/mceph mice were incubated with an antibody against Kv1.2.
For in vitro expansion of peptide-specific T cells, splenocytes from immunized mice were incubated in the presence of the selected peptides (5 µg/ml).
For CFSE (Molecular Probes) labeling, lymph node cells from TCR-transgenic CL4 mice were incubated with 2.5 µM CFSE (10' RT) and then centrifuged through a FCS cushion.
Serial two fold dilutions of heat inactivated sera from infected mice were incubated with VACV-WR for 60 minutes at 37c.
