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Founder mice were identified by PAGE-based PCR genotyping from tail biopsies34.
Doubly mutant mice were identified by genotyping.
Transgenic mice were identified by PCR.
Founder mice were identified as described [17].
In following generations, homozygous Sost KO and littermate control (LC) mice were identified using a Multiplex PCR with mice tail cuts, according to a protocol provided by Novartis.
Autophagic bodies were identified inside of cells exposed to VCC [52].
Consistently, multiple recombination events were identified inside these gene clusters.
P-DIE and P-SUR mice were sacrificed in pairs (n = 11/each group) whenever a P-DIE mouse was identified.
Interestingly, Sca1-positive cells could be identified inside blood vessels in tissue sections of the grafted tumor in WT mice injected with lung tissue cells from a 70-day-old MMT donor mouse.
The putative catalytic triad Asp802-Glu831-Asp919 was insidefieachnside each of the catalytic domains (Fig. 1C).
The vector P. argentipes and the parasite L. donovani have both been identified inside the town.
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