Brains from 7-day-old C57BL/6J mice were homogenized in homogenization buffer (0.32 M sucrose, 50 mM Tris-HCl (pH 7.4), 1 mM CaCl2, and 1 mM MgCl2, 1 mM phenylmethylsulfonyl fluoride (PMSF), Complete Protease Inhibitor Cocktail, EDTA-free (Roche Diagnostics, Mannheim, Germany), and Phosphatase Inhibitor Cocktail I (Sigma)).
Kidneys of control and furosemide-treated mice were homogenized in homogenization buffer A (HbA; 20 mmol/L Tris/HCl (pH = 7.4), 5 mmol/L MgCl2, 5 mmol/L NaH2PO4, 1 mmol/L EDTA, 80 mmol/L sucrose, 1 mmol/L phenylmethanesulfonylfluoride, 1 μg/mL leupeptin and 10 μg/mL pepstatin).
Hearts from WT and ecSOD Tg mice were homogenized and sonicated in ice-cold homogenization buffer containing (in mM) 20 HEPES, 1 EGTA, 210 mannitol, and 70 sucrose.
Embryos isolated from 10 12 day pregnant C57Bl6 Lrp5 +/+ or Lrp5 −/− mice were homogenized for 1 min/4°C in "Streuli" lysis buffer [74] using a Polytron homogenizer.
Retinal tissue from PARP1 KO, wt and rd1 mice were homogenized in buffer as described previously [7] with a Heidolph DIAX 600 homogenizer (Heidolph, Schwabach, Germany) or a manual homogenizer (glass to glass).
Four different brain regions from wild-type or parkin knockout mice were homogenized in lysis buffer containing 10 mmol/L Tris HCl [pH 7.4], 150 mmol/L NaCl, 5 mmol/L EDTA, 0.5% Nonidet P-40, Phosphate Inhibitor Cocktail I and II (Sigma), and Complete Protease Inhibitor Mixture (Roche), using homogenizer.
E18 brains from either PME-1 or mice were homogenized in Trizol (Invitrogen) and proteins isolated following the manufactureŕs directions.
To determine LM colony forming units (CFUs), spleens and livers from infected mice were homogenized in sterile double distilled H2O.
Skin samples of mice were homogenized in 500 µl 0.05% hexadecyltrimethylammonium bromide (HTAB in 0.05 M phosphate buffer, pH 6; Sigma) solution.
Bladders and right side kidneys from 5 infected mice were homogenized in sterile PBS containing 0.02% triton-X 100, and serial dilutions were plated on LB agar plates.
For foci assay, lungs of mice were homogenized in phosphate buffered saline (PBS) with 0.1% BSA using the PolyTron 2100 homogenizer.
