Before water maze training, mice were handled for 5 min per day over 5 consecutive days.
Forty male C57BL/6J mice were handled by the experimenter or received minimal exposure to human contact.
All mice were handled once per day for two days prior to experiments, in order to accustom the animals to the experimenter.
When the mice were handled, it was with the same kind of surgical glove, and when they were tested it was on the same equipment, at the same time in the morning.
You'll be glad to know that these researchers were not allowed to traumatize their subjects, unlike how the Swiss mice were handled.
All mice were handled daily to habituate them to experimental maneuvers.
For five days before behavioral analysis, the mice were handled daily.
The mice were handled and maintained using micro isolator techniques, with daily veterinarian monitoring.
All mice were handled gently for 5 consecutive days prior to the injections to minimize stress associated with injections.
All mice were handled according to protocols approved by the University of Virginia Institutional Animal Care and Use Committee.
In all studies, mice were handled and euthanized in accordance with approved institutional, national and international guidelines.
