The lung tissues removed from the infected mice were fixed with 2.5% glutaraldehyde and post-fixed in 1% osmium tetroxide.
Segments of the distal colon from mice were fixed in water-free Methanol-Carnoy's fixative [60% dry methanol, 30% chloroform and 10% acetic acid].
Briefly, adult mice were fixed with paraformaldehyde; the muscles were removed, post-fixed, rinsed and mounted on slides.
For histological analysis, liver tissue specimens from CCl4-injuried mice were fixed in 4% PFA overnight, then dehydrated by graded ethanol at RT and embedded in paraffin.
Limbs of P1 mice were fixed with 4% PFA overnight.
The mice were fixed at P12, and the lens and vitreous body were removed.
Sperm suspensions from hyh and wild type mice were fixed in 2% paraformaldehyde.
The MLNs of all mice were fixed in a solution containing paraformaldehyde 0.5% and glutaraldehyde 0.5%.
For histological analysis, limbs from 8 week old mice were fixed in formalin for 48 h.
Inner ears of C57BL/6J new born mice were fixed in 4% paraformaldehyde (PFA).
Eyes from 2, 14, 28, 42 day old mice were fixed with 2% paraformaldehyde and paraffin embedded.
