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WT or DUSP4-/ mice were first treated with NAC for two weeks, and subsequently hearts were isolated and subjected to 30 min ischemia and 30 min reperfusion.
Prior to performing the injections into tumors, mice were first lightly anesthetized with isoflurane gas.
For adoptive transfer studies, both Jak2−/− and control mice were first underwent 800 rads of irradiation.
The femora (n = 14 mice) were first scanned using in vivo μCT as described below.
Ctgf Lo/+ mice were first intercrossed to determine if the Ctgf Lo/Lo phenotype is viable.
From the age of 5 months, mice were first trained on the rotarod device before testing.
To evaluate the ability of NKp30-Ig to enhance macrophages-mediated lysis of tumor cells, mice were first injected (i.p).
For this purpose, murine embryonic fibroblasts (MEF) derived from Hpse-KO and wt mice were first examined for MMP expression.
Water-deprived mice were first trained to distinguish between a high concentration (10−3) of - carvone and mineral oil.
The donor BALB/c mice were first immunized with DNCB or vehicle for three or four times, as described above.
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Excised mammary glands from GFP-mice were first examined using conventional bright field with a stereofluorescence microscope.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com