But Algernon, the mouse who was experimented on before Charlie, loses his intelligence and dies, and Charlie, now a genius, foresees what lies ahead for him.
Next day, newspaper carried report that the dentists were experimenting on a mouse with pyorrhea.
Meanwhile, researchers are experimenting on mice, trying to isolate the mechanisms that cause aging, in an effort to slow (or stop) them.
The conclusions from these experiments on mice were supported by experiments in vitro with human cells showing similar results [ 61].
The mice were sacrificed after the experiment on the 23rd day, and parts of Achilles tendons were then used for histological verification.
In the second experiment, the mice were sacrificed for dissection on day 21 after cell injection.
In one such experiment, mice were sacrificed on day 25 for histological examination of the joints.
All experiments on mice were performed in accordance with Edinburgh University rules and National Guidelines on animal experimentation.
In the first experiment, mice were maintained on As for 5 weeks, and in the second, repeat experiment, mice were maintained on As for 6 weeks.
Overall, five mice were excluded from the experiment based on the altered motor ability; three from the regular food+rotenone group and two from the regular food+vehicle group.
At least 3 animals for every time point, for all feeding types and for each genotype (only male mice were used in all experiments, female mice were used only for one experiment shown on Supplementary Figure 2) were used for all experiments.
