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For Treadmill test functional assay, 4-week-old mice were exercised three times, once a week, before recording of their performances.
The mice were exercised until they displayed signs of exhaustion (>2 s spent on the shocker plate without attempting to re-engage the treadmill) or the exercise was terminated after 60 min. The second group of mice was subjected to a long-term exercise stress.
To promote muscle pathology, mice were exercised for 30 minutes twice a week.
After treadmill acclimatization, mice were exercised for 30 min three times per week for two weeks.
For EBD uptake, mice were exercised on a treadmill (MK-680S, Muromachi Kikai) as described (34).
Mice were exercised daily, 5 days per week.
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Next, the intensity chosen for the exhaustive exercise (at CS) was individually related to performance (at around 80% of vPeak); thus, all mice were exercising at the same relative intensity.
To analyze the effects of different exercise bouts, one group of WT and VLCAD−/− mice was exercised for 60 min once.
10 wildtype mice were exercise trained at low impact (<10 m/min) on a Exer-6 M treadmill (Columbus Instruments) following acclimatization every other day for one week to increase treadmill time from 1 hour to 2 hours.
Even before Meijer got creative in her yard, researchers knew that captive mice are exercise maniacs.
Dy3K/δE3 mice (n = 4) were exercised for 30 min on a treadmill Exer 6M (Columbus Instruments) at a downhill angle of 15°.
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