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Raptor overexpressed mice were established by cardiac-specific injection of adenovirus specified in Supplementary Material).
Similarly, xenografts (LU139 and H2171 cells in NSG mice; H146 cells in ICR SCID mice) were established by subcutaneously injecting 100 µL of cells at a concentration of 10e6 cell per mouse also into the right flank of each mouse.
Transgenic mice were established carrying human Cx26 with the R75W mutation that was identified in a deaf family with autosomal dominant negative inheritance [Kudo T et al. (2003) Hum Mol Genet 12 995 1004].
Humanized mice were established by transplanting human CD34+ cord blood cells into irradiated severely immunodeficient NOD/Shi-scid/IL2Rγnull (NOG) mice, and the phenotypes of human cells contained in bone marrow, thymus, spleen and peripheral blood from the mice were analyzed at monthly intervals until 4 months after cell transplantation.
Hcy treated mice were established to investigate the in vivo role of Hcy in regulating T cell mitochondrial metabolism.
Metastatic lesions in Balb/c mice were established by intracardiac injection of luciferase-expressing 4T1luc mouse breast carcinoma cells.
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Such investigations are more easily performed in the laboratory, and to this end, a tumor model in nude mice was established to simulate several aspects of the postmenopausal breast cancer patient.
In virulence studies, a group of ten immuno-suppressed mice was established for each yeast strain.
A colony of YG8R B6.Cg-FxnTg1Mkn Tg(FXN YG8Pook/J mice was established at UC Davis (Jackson Labs, # 012253) (68).
The specificity of antibodies elicited in immunized mice was established in immunoblots using EAC TCLs as antigens.
We therefore examined whether increased lymphatic vessel density in ACKR2-deficient mice was established during embryogenesis.
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