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In the first 225Ac-E4G10 monotherapy study, mice were engrafted with 1 million LNCaP cells.
Athymic locally bred Swiss nude mice were engrafted with 6×105 of NB8-E6 (8 animals), NB8-CXCR4-C3 (8 animals), N91-E2 (8 andmals), aN91-CXCR4-14-14 (13 aninals) cells in 15 µl DMEM in the left adrenal gland.
Per human donor, three mice were engrafted and six independent donors were used for transplantation.
NOD SCID mice were engrafted with 5 × 106 MDA-MB-231 cells by subcutaneous injection in one hind flank.
NSG mice were engrafted with either 1 × 10 SiPKR or Sicontrol cells that express a luciferase reporter.
Athymic locally bred Swiss nude mice were engrafted with the IGR-N-91 cell line, in accordance to the European Community guidelines (authorization #86/609/CEE).
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The humanized mouse model, in which immunodeficient mice are engrafted with human hematopoietic cells or tissues, or mice that transgenically express human genes [ 35, 36], could be an alternative to deal with the problem of the mismatch between mouse and human MHC.
In order to determine whether CD8 T cells in humanized mice are able to respond to A2 restricted virus-specific peptides previously identified in humans, we assessed IFN-γ responses in NOD-scid IL2rγnull mice that were engrafted with HLA A2 cord blood cells.
Depletion of B cells with rituximab in severe combined immunodeficiency mice that were engrafted with human synovium from patients with RA significantly suppressed the production of interferon-γ and several macrophage-derived cytokines such as tumor necrosis factor-α and interleukin-1β [ 41].
Cells from all patients were engrafted, and engraftment was documented in 44 of 46 evaluable mice (95%).
NOD/SCID mice (n = 5/group, male) were engrafted subcutaneously with the mixture of SKOV3 cells (2 × 106) and fresh isolated human PBMCs (1 × 107).
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