To assess proliferation of cell subpopulations in our models, sections of E3, MCF-7, and EWD8 bone metastases from E- or E+P-treated mice were dual-stained for CK5 and the mitosis marker pHH3 [ 36].
Then cells were dual stained with mouse anti-vimentin (1 200; Sigma) and guinea pig anti-keratin (1 100; Sigma) at 4°C overnight.
However, both were dual gauge.
Cells injected into the #4 (left side) mammary fat pad of mice were labeled with dual-luciferase-Tomato red reporter gene (pFULT) vector and cells injected into the #9 (right side) mammary fat pad of mice were labeled with dual luciferase-GFP reporter gene (pFULG) vector.
In this study, we report CNX-012-570 (whash han an half-life of 5 h in mice) is a dual β1 and β2 subunit specific potent and direct AMPK activator for its potential to control multiple aspects of metabolic syndrome in both diet-induced obese (DIO) and db/db mice.
In addition, mice were also created with dual expression of SR-BI exclusively in endothelium (Tie2- Scarb1 transgene) and liver (human SCARB1 gene under control of a liver-specific promoter) in mice with the Scarb1 knockout background.
One month-old wild-type C57BL/6 mice were injected with the dual AAV vectors (dose of each vector/eye: 1.7 × 10 GC) and eyecup lysates were evaluated 1 month later using Western blot analysis with anti-HA antibodies.
To evaluate the biological and therapeutic activity of the recombinant ABCA4 protein produced by dual AAV vectors, 1 month-old albino Abca4−/− mice were injected subretinally with the dual AAV TS and hybrid AK RHO- ABCA4-HA vectors (dose of each vector/eye: 1 3 × 10 GC).
Following euthanasia, female mice were subsequently scanned using dual-energy X-ray absorptiometry (DEXA) with a Lunar PIXImus2 Densitometer (GE Medical Systems; Madison, WI, USA).
Apo E−/− mice were intravenously injected with dual-targeted PV-MPIO or negative control IgG-MPIO (30 mg iron per kg body weight) and allowed to circulate for 30 min.
For fecal dual isotope experiments, mice were starved overnight and then received a gastric bolus of 100 µl cornoil containing 1 µCi 14C-Oleic Acid and 1 µCi non-absorbable 3H-Sitostanol.
