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Handling and testing of the mice were done during the light phase of the light-dark cycle.
Statistical comparisons between AMD3100-treated mice and PBS-injected mock mice were done using the Student t test.
These studies on the KO and D36 mice were done in parallel and therefore cannot be explained by different experimental conditions.
Statistical analysis between the different groups of vaccinated and unvaccinated mice as compared to the PBS control mice were done using Fisher's exact test.
Injections of AAV-Cre or AAV-GFP into the rPCRt of these mice were done using a compressed air delivery system [37], [41].
Analysis of CD41 positive platelets in kidney from healthy mice were done by manual counting of all positively stained platelets in sections from whole kidney from n = 5 6 individuals/group.
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Blood samples collection of mice was done by T.Y.Z. and Q.Y.
Genotyping of the knock-in mice was done by PCR.
The generation of the 3xTg-AD mice was done as previously described [30], [31], [37].
Routine identification of transgenic mice was done by PCR [61], [62].
The enrichment of CD11c+ DC in mice was done according to the previous protocols reported [27].
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CEO of Professional Science Editing for Scientists @ prosciediting.com