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Brains and spinal cords from transgenic GFAP-EGFP mice were dissociated using the papain-based neural tissue dissociation kit (Miltenyi Biotec, Germany).
Tumors from transgenic mice were dissociated as described in Kim, Roopra and Alexander (submitted).
MLNs from WT and galectin-3−/− mice were dissociated and the cells were cultured in RPMI 1640 medium supplemented with 10% SFB in 12-well plates (Corning, USA) for 2 h at 37°C and 5% CO2 atmosphere.
For primary astrocyte cultures, the cerebral cortices of postnatal day 1 mice were dissociated using trypsin (GIBCO), and cultured for two weeks in DMEM containing 10% fetal calf serum.
In an effort to quantify the effect of transgenic expression of DKK1 on the frequency of total TECs and specific TEC subsets, thymic lobes from TetO-Dkk1 and K5rtTA-ST littermate mice were dissociated using Collagenase/Dispase/DNase digestion following 4 weeks of Dox feeding.
Cerebral cortical cells from newborn mice were dissociated by trypsinization and plated in complete DMEM.
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ScW from 12-week-old Bmp8b WT and TG mice was dissociated by collagenase treatment isolating unilocular adipocytes from the stromavascular fraction (SVF).
ScW from 12-week-old Bmp8b WT and TG mice was dissociated by collagenase treatment isolating unilocular adipocytes from the SVF.
BMM were isolated from femur bone marrow of C57BL/6 mice, and were dissociated into single cell suspensions.
The natural segregation of human adult cell types and the emergence of multi-cellular formations in co-cultures mimicked studies where fetal mouse lungs were dissociated and cultured [13], [14].
Rat and mouse islets were dissociated into single cells and purified by FACS into beta cells (mean purity 90% insulin-positive cells) and alpha enriched-cells (75% glucagon-positive cells, 25% insulin-positive cells) using cellular light-scatter and FAD-autofluorescence as discriminating parameters.
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