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Spleens from NZW mice and NZB mice were disaggregated and erythrocytes were lysed (Puregene RBC lysis solution; Gentra Ltd, Minneapolis, MN, USA).
Spleens from 6- to 10-week-old CBAB6F1 mice were disaggregated and the single-cell suspension was centrifuged on a Ficoll cushion to remove erythrocytes and cultured for 7 days in RPMI supplemented with 15% FCS, 50 μM 2-mercaptoethanol, 50 μg/ml gentamicin, 25 μg/ml LPS, 5 ng/ml IL-5, 5 ng/ml IL-6 and 10 ng/ml IL-10.
Similar(58)
Briefly, serially passaged solid human xenografts growing subcutaneously in nude mice (NMRI nu/nu strain) were disaggregated, and 4 × 104 to 8 × 104 viable cells were added to 0.2 mL of Iscove's medium (supplemented with 20% vol/vol FCS and 1% vol/vol gentamicin) containing 0.4% agar and plated on top of the base layer (0.75% agar).
Lymph nodes were disaggregated by treatment with 0.1% collagenase D (Roche) at 37°C for 1 hour.
Healthy ICM outgrowths were disaggregated between day 7 and 8, and plated onto fresh feeder cells.
The epidermal cells were disaggregated and fixed in 1% formaldehyde for 15 mins at RT.
Once confluent, cells were disaggregated from each flask using trypsinisation.
Feeding data were disaggregated by season based on farmer recall.
Tissues were disaggregated by rubbing between frosted glass slides.
After treatment, MCC were disaggregated with trypsin and washed.
MCF-7 cells were disaggregated and resuspended in complete medium.
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