Sentence examples for mice were digested from inspiring English sources

Tumor tissues of G3- and vector-expression cell treated mice were digested and lysated.

To genotype mice, tail biopsies from 3 week old mice were digested overnight at 55°C in Direct PCR Tail lysis buffer (Qiagen) and proteinase K (Qiagen).

For osteoblast cultures, calvariae of neonatal mice were digested by 0.1% collagenase and 0.2% dispase 5 times, and cells isolated by the last 3 digestions were combined and cultured in α-minimal essential medium (α-MEM) containing 10% FBS.

Livers from wild type and MMTV-Cre mice were digested in buffer containing 2 mg/ml protease K, 10 mM Tris pH 8.0, 25 mM EDTA, 0.5% SDS, 100 mM NaCl at 55°C overnight.

The proximal region of prostatic ducts (i.e., the portion of the ducts nearest the urethra) of 6 week old C57BL/6 mice were digested and cells were examined for antigen expression (Table S1) [2], [2].

In brief, lungs from neonatal ACTN4 mutant and wild type littermate mice were digested for 45 min at 37°C in RPMI with 0.28 U/ml liberase blendzyme 3 and 60 U/ml DNase I, passed through a 70 µm filter, centrifuged at 540 x g at 4°C, and plated in tissue culture flasks in DMEM with 15% fetal bovine serum (FBS).

Approximately 0.5 mg of DNA from various treated mice was digested and analyzed for titanium content.

10 20 µg genomic DNA from the tails of mice was digested with the indicated restriction enzymes and analyzed by Southern blot using a-P32-dCTP labeled random-primed DNA probes (Stratagene Prime-It II kit).

To sequence the transgenic insertion at the Metrn locus, genomic DNA from tgε26+/+ mice was digested with NcoI, self-ligated, and amplified using inversely oriented PCR primers (Fig. 2H, i-1F, i-1R, i-2F, and i-2R).

CKm purified from middle-aged and aged mice is digested approximately 3.5 times faster than CKm purified from young mice.

For NAD(P H measurements from primary L cells, SI tissue from GLU-Venus mice was digested to single cells [ 7] and analysed on the same day.