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The necks of the mice were cut, and the tissues surrounding the trachea were removed.
Rando's experiments involved an unsettling but remarkable procedure in which mice were cut along the flanks and sewn together, wound-on-wound.
Livers from individual mice were cut into 2 × 4 × 4 mm3 sections, fixed in 4% paraformldehyde and embedded in paraffin.
The brain was mounted and coronal slices (500 µm for rat, 250 µm for mice) were cut using a vibratome.
WAT isolated from three month old mice were cut into small pieces and 8 days post differentiated MEF were used for lipolysis assay [45].
Tumor tissues harvested at 38 days post tumor implantation from control, Noscapine, Doxorubicin and combination treated mice were cut into small pieces and homogenized in PBS.
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The cerebellum from PND12 mice was cut into 10 µm sagittal sections and collected at 50-section intervals.
Freshly isolated PVAT from WT and AKO mice was cut into two pieces of 5 15 mg.
Under isoflurane (2%) anesthesia, a unilateral L4 spinal nerve of mice was cut as described previously (Tsuda et al., 2009).
Cecal tissue isolated from BALB/c and AKR mice was cut longitudinally, washed with phosphate-buffered saline, and kept hydrated in a 6-well plate.
Briefly, the thoracic aorta, isolated from control and mildly arthritic mice, was cut into rings of 2 mm length and mounted in separate wells of a Mulvany myograph (Danish Myo-Technology, Aarhus, Denmark) and bathed in Krebs buffer (composition (mM): NaCl 118, KCl 4.7, KH2PO4 1.2, MgSO4.7H2O 1.2, NaHCO3 25, glucose 11, CaCl2·7H2O 1.5) at 37°C gassed with 95%O2/5%% CO2.
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CEO of Professional Science Editing for Scientists @ prosciediting.com