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hUGT1/Car−/− mice were constructed previously23.
BM chimeric mice were constructed using the method described by Zhou et al.52 Briefly, following exposure to an X-ray dose of 5 Gy × 2, miRNA223−/+ and WT-C57BL/6J mice received an intravenous administration of BM cells (approximately 4 × 106 cells) from miRNA223−/+ or WT-C57BL/6J mice not exposed to X-ray.
Human fetal liver and thymus SCID-hu mice were constructed as previously described [18], [25].
SCID-hu and SCID-rab mice were constructed as previously described [34], [35].
Additionally, bone-marrow chimeric mice were constructed to elucidate the contribution of the inflammatory response to cardiac damage.
VMD2-IL-10 transgenic (Tg) mice were constructed to overexpress IL-10 (pVMD2-placF was provided by Noriko Ezumi, Johns Hopkins Medical School).
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A subcutaneous tumor model of nude mice was constructed.
The plasmid to create Sycp1-Cre Tg mice was constructed from two fragments.
A Lashley III maze, scaled for use with mice, was constructed from black Plexiglas and located in a dimly lit room (10 Lux at the floor of the maze).
The care and use of laboratory animals have been approved by the Tsinghua University Animal Care and Use Committee, complying with the rules of REGULATIONS FOR THE ADMINISTRATION OF AFFAIRS CONCERNING EX-PERIMENTAL ANIMALS (Approved by the State Council of P.R. China) A subcutaneous tumor model of nude mice was constructed.
In the BXD cross, an F2 population consisting of 111 mice was constructed from a cross of two inbred strains of mice, C57BL/6J and DBA/2J [ 10, 15].
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