Mice were sacrificed when tumors reached an average of 2000 mm3 and mice were considered not survivors.
The mice were considered immobile when they passively hung or stayed motionless [26].
For analyses, only those protein spots which were changed in intensity in both mice were considered to be significantly altered.
If glucose levels were more than 250 mg/dl, these mice were considered to have the mutation.
For the Kaplan-Meier tumor free survival curves, mice were considered tumor free until tumors were visible or palpable.
If the beam at the edge of the open field, 1 cm apart from the wall, was not interrupted, mice were considered to be in the center area.
Blood glucose was monitored weekly until 30 weeks of age, and mice were considered diabetic when glucose levels were >300 mg/dl for two consecutive weeks.
After ten weeks, reconstitution levels were evaluated by assessing Ly 5.2 and Ly 5.1 expression in blood and mice were considered reconstituted when hematopoietic cells were >80% donor-derived.
Sera from peptide-immunized mice were considered significantly positive for native PrP binding when the mean fluorescence intensity (MFI) was greater than the MFI+3SD obtained with sera from normal mice.
None of the mice benefited from the administration of Ad-β-gal or vehicle as all the tumors reached 2 cm3 when mice were considered not survivors (Figure 4A and Figure S6).
The majority of non-neoplastic and neoplastic lesions observed in the Pol β Tg mice were considered spontaneous and age-related as previously reported in C57BL and other mouse strains [50], [60] [63].
