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First, a group of mice were conditioned using 5 mg/kg cocaine (Fig. 2).
129S6 mice were conditioned sequentially in two different environments (design illustrated in Fig. 8A).
In this test, mice were conditioned to associate an auditory cue or environmental contexts with a foot shock.
Briefly, newborn mice were conditioned by irradiating with 350 rads and then injected intrahepatically with 0.5-1×106 human CD34+ cells.
Thus, a group of mice were conditioned to cocaine, had this place preference extinguished and were then tested for primed reinstatement of the original place preference.
During days 2 5, mice were conditioned twice per day in 15 min sessions spaced four hours apart (session 1∶1000 h; session 2∶1400 h).
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We found that all three genotypes of mice developed a similar degree of conditioned place preference to this dose (F 2,23) = 0.74, n.s).. To determine if mGluR5 heterozygous or knockout mice had enhanced sensitivity to the conditioned reinforcing effects of a low dose of cocaine, another group of mice was conditioned with 1 mg/kg cocaine.
On day 2 (the training day), each mouse was conditioned in a 10 minute session during which sudden air blows were delivered whenever the mouse visited one of the randomly assigned chamber (termed air-puff chamber).
On the other hand, offspring of mice that had been conditioned to fear another smell, or mice who'd had no such conditioning had no fear of cherry blossom.
A total of 18 mice were trace conditioned, and 17 mice pseudo-conditioned.
Thus, mice were successfully conditioned to avoid the taste of NaCl after self-administration of 150 mM LiCl in 2-bottle conditioning sessions.
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