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Lastly, despite being hypermetabolic, VGF knockout mice were cold intolerant.
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At 5 days post injection, mice were cold-challenged (4 °C/75 min) and killed.
Although Dbh −/− mice are cold sensitive due to lack of brown fat thermogenesis, they can shiver normally [9].
Both ob/ob mice, which have very little UCP1 [24], and UCP1 −/− mice are cold sensitive if immediately exposed to 4°C, but can tolerate this temperature if they undergo a cold adaptation protocol which allows time to increase shivering capacity [25], [26].
Briefly, mouse pups at P7 were "cold" anesthetized by placement on ice.
When the mice were in colder temps, the cells lining their nose were markedly worse at fighting of the virus.
Wild-type (Wt, white bars) mice and GPR120−/− mice (grey bars) mice were exposed to cold (4 °C) for 7 days (n=5).
(A D) The wildtype mice were subject to cold challenge.
(A E) The wildtype mice were subject to cold challenge.
(A and B) The wildtype mice were subject to cold challenge.
The wildtype mice were subject to cold challenge, and then recovered at thermal-neutral condition (32 °C).
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