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Using the liquid diet method, mice were chronically treated with skimmed milk containing 5% ethanol for 5 days.
Under pentobarbital anesthesia (50 mg/kg, intraperitoneally) mice were chronically implanted with EEG and EMG electrodes for polysomnography, as previously described25, 26.
A total of 21 male apolipoprotein-E-deficient mice were chronically infused with angiotensin II (1.44 mg/kg daily) for 28 days to induce AAA formation.
Naive mice were then chronically treated with vehicle (Naive + Veh) and alcohol-exposed mice were chronically treated with either vehicle (EtOH + Veh) or Tandospirone (3 mg/kg/day) (EtOH + Tando), for 2 weeks.
Young male and female Swiss Albino mice were chronically as well as transgenerationally exposed to aqueous extract of betel nut (AEBN) in drinking water (2 mg ml−1) for up to 24 weeks.
The content of copper (Cu) NPs in the olfactory bulb increased after the mice were chronically exposed to Cu NPs through intranasal instillation [33, 34].
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When mice are chronically stressed — by sudden changes in their living environments or by the removal of their bedding — they demonstrate behavioral symptoms like anxiety and lethargy and lose their sense of adventurousness, features that mimic aspects of human depression.
In this model the subordinate mouse is chronically stressed by the unavoidable sensory contact with the dominant conspecific.
Studies in a certain line of genetically modified mice (i.e., HCV core transgenic mice) that were chronically fed alcohol found that TLR4 activation leads to up regulation of Nanog in stem cells (Machida et al. 2009).
JS1 cells (mouse activated HSCs) and primary HSCs from mice that were chronically administered CCl4 for 1 month were a gift from Drs. Scott Friedman, Virginia Hernandez-Gea and Zahra Ghiassi-Nejad.
In the present study, we show that PARP-1 is activated in human asthma and that PARP inhibition genetically or by olaparib blocks chronic asthma traits in mice that were chronically exposed to HDM extract.
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