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The mice were boosted three times at one-month intervals.
Fourteen days after primary immunization, mice were boosted with their respective antigens.
After 2 weeks, mice were boosted with the same amount of protein with incomplete Freund's adjuvant.
CFA recipient mice were boosted with their respective antigens emulsified in IFA (Sigma-Aldrich).
At week 30, all mice were boosted i.m. with dose of 0.5 µg HA H5N1 non-adjuvanted inactivated virus vaccine.
On Day 30, all mice were boosted via injection of the same immunogens into their left, rear footpads (Fig. 3A).
Thereafter, the mice were boosted twice with 50 µg Ndk-Alum mixture after intervals of 14 days.
At day 80 after priming mice were boosted and the IgG anti-NIP titer was determined at day 94.
At day 20 after primary immunization, mice were boosted s.c. with 100 µg of CII in CFA.
At week 30, all mice were boosted with dose of 0.5 µg HA H5N1 non-adjuvanted inactivated virus vaccine.
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Some DNA-primed-mice were boosted with Ag85B protein twice at 2-week intervals (NP-mice).
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