At the end of the study, mice were sacrificed by CO2 inhalation; mice were bled via cardiac puncture, and blood sampled into EDTA tubes.
One hour later, the mice were bled by cardiac puncture, and the pulmonary vasculature was flushed by right ventricle puncture.
Five days after each immunization, 10 mice were bled from the tail vein, and the serum was isolated and stored at −20 °C until use.
The mice were bled at 6 and 10 weeks after inhalation, and the number of antibody molecules of each isotype present on their RBC was measured by DELAT.
The mice were bled on day 40.
On day 49, the mice were bled.
After resting for two weeks the mice were bled (day 28).
Three weeks post-immunization the mice were bled by cardiac puncture and sacrificed.
Ten days after the last immunization, mice were bled from the ocular venus plexus.
Following the single vaccination, all mice were bled at 3 weeks and also at 2 months.
At day +7 post transplantation, the mice were bled and plasma prepared.
