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The normalized brains of all the mice were averaged to create a custom template.
The sums of the luciferase counts of all organs from single mice were averaged for each strain and compared between strains (Figure 1c).
Escape latencies for individual mice were averaged from each daily trial and for the time the animals needed within the first trial per day to reach the platform.
Photopic b-wave maximum amplitudes (those generated at 12 cds/m2) of all smCBA-mGC1 treated (n = 6) and hGRK1-mGC1 treated (n = 8) GC1KO (both treated and untreated eyes) and isogenic +/+ control mice were averaged and used to generate standard errors.
The cell counts from all of the sections of all of the mice were averaged.
Proximal and distal colitis scores from individual mice were averaged, unless otherwise stated.
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The fold change of virus-induced gene expression levels in PT-treated mice compared to control mice was averaged from the three mice per group, and averaged gene expression data was filtered using a cut-off value of 1.5-fold up- or down-regulation and a statistical significance of P<0.05.
The speed of each of the vocalizing mice was averaged to determine the mean instantaneous speed, which was then averaged across a recording session for all vocal sequences.
Results from both EDL muscles of one mouse were averaged to give a single data point.
The errors for the last 3 days of testing of each mouse were averaged and used for statistical analysis.
The latency to fall (seconds) was recorded and all six scores per mouse were averaged and recorded as latency to fall (in seconds) for each mouse.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com